Measuring spectral extinction with digital holography.

The optical extinction caused by a small particle, such as an aerosol particle, is an important measurable quantity. Understanding the influence of atmospheric aerosols on the climate, assessing visibility in urban environments, and remote sensing applications such as lidar all need accurate measurements of particle extinction. While multiple methods are known to measure extinction, digital in-line holography (DIH) features the unique ability to provide contact-free images of particles simultaneously with estimates for the extinction cross section. This is achieved through an integration of a measured hologram followed by an extrapolation. By means of a supercontinuum laser, we investigate the measurement of the cross section via DIH for stationary particles across a broad spectrum, from 440 nm to 1040 nm. The particles considered include a 50 µm glass microsphere, a volcanic ash particle, and an iron(III) oxide particle. The results show the ability to estimate a particle's cross section to within 10% error across portions of the spectrum and approximately 20% error otherwise. An examination of the accompanying hologram-derived particle images reveals details in the images that evolve with wavelength. The behavior suggests a basic means to resolve whether absorption or scattering dominates a particle's extinction.

Measuring extinction with digital holography: noisy holograms.

Digital in-line holography is a versatile method to obtain lens-less images of small particles, such as aerosol particles, ranging from several to over one hundred microns in size. It has been shown theoretically, and verified by measurement, that a particle's extinction cross section can also be obtained from a digital hologram. The process involves a straightforward integration, but if noise is present it fails to give accurate results. Here we present a method to reduce the noise in measured holograms of single particles for the purpose of rendering the cross-section estimation more effective. The method involves masking the complex-valued particle image-amplitude obtained from a noisy hologram followed by a Fresnel transformation to generate a new noise-reduced hologram. Examples are given at two wavelengths, 440 nm and 1040 nm, where the cross section is obtained for a micro-sphere particle and several non-spherical particles approximately 50 microns in size.

Black carbon aerosol number and mass concentration measurements by picosecond short-range elastic backscatter lidar.

Black carbon aerosol emissions are recognized as contributors to global warming and air pollution. There remains, however, a lack of techniques to remotely measure black carbon aerosol particles with high range and time resolution. This article presents a direct and contact-free remote technique to estimate the black carbon aerosol number and mass concentration at a few meters from the emission source. This is done using the Colibri instrument based on a novel technique, referred to here as Picosecond Short-Range Elastic Backscatter Lidar (PSR-EBL). To address the complexity of retrieving lidar products at short measurement ranges, we apply a forward inversion method featuring radiometric lidar calibration. Our method is based on an extension of a well-established light-scattering model, the Rayleigh-Debye-Gans for Fractal-Aggregates (RDG-FA) theory, which computes an analytical expression of lidar parameters. These parameters are the backscattering cross-sections and the lidar ratio for black carbon fractal aggregates. Using a small-scale Jet A-1 kerosene pool fire, we demonstrate the ability of the technique to quantify the aerosol number and mass concentration with centimetre range-resolution and millisecond time-resolution.

Multispectral small-angle light scattering from particles.

Using a supercontinuum laser, reflective optics, and a spatial filter, we measure two-dimensional small-angle light-scattering patterns for a variety of microparticles including spheres, salt, sand, and volcanic dust. The measurements are done at 13 wavelengths from 450-850 nm, where the absence of refractive optical elements minimizes the effects of chromatic aberration. Qualitative particle-material sensitivity is demonstrated by layering differently colored patterns. Last, the multispectral capability of our device demonstrates a new possibility to probe different q-space regimes for a given particle in a single measurement.

Two-dimensional small-angle scattering from single particles in infrared with a lensless technique.

An experiment is described where two-dimensional small-angle light scattering (2D-SALS) patterns from single particles are measured in the infrared through a lens-free approach. Spatial filtering is employed to separate scattered light from unscattered light to within approximately one degree from the forward direction. Non-planar reflective elements are used in the filtering process, permitting 2D-SALS measurements to be done without chromatic aberrations over a broad spectral range and from 0.8 to 8 degrees in the polar scattering angle and zero to 360 degrees in the azimuthal angle. Patterns from spherical microparticles are presented along with nonspherical particles including volcanic ash and salt. An asymmetry analysis is applied to demonstrate an ability to differentiate spherical from nonspherical particles from the 2D-SALS patterns.

Spectralon spatial depolarization: towards an intrinsic characterization using a novel phase shift distribution analysis.

Spectralons are reference radiometric samples which exhibit a calibrated reflectance. However, in case of low reflectance samples, the degree of polarization (DOP) of scattered light is hard to characterize. Here, an accurate determination of spectralon spatial depolarization is proposed. Based on a spatially resolved polarimetric imaging system, the polarization state of the scattered light is characterized for every pixel. A statistic distribution analysis is carried out over the entire image. The relative phase shift distribution between two orthogonal components of the scattered electric field clearly exhibits a high sensitivity to the reflectance, the phase statistics following a circular Voigt profile. The intrinsic part of the spatial depolarization is demonstrated to be linked to the circular Cauchy contribution of that phase dispersion. An analytic equation is proposed to estimate the monochromatic spatially integrated DOP, as a function of the reflectance.

Imaging tissue-mimic with light sheet microscopy: A comparative guideline.

Tissue mimics (TMs) on the scale of several hundred microns provide a beneficial cell culture configuration for in vitro engineered tissue and are currently under the spotlight in tissue engineering and regenerative medicine. Due to the cell density and size, TMs are fairly inaccessible to optical observation and imaging within these samples remains challenging. Light Sheet Fluorescence Microscopy (LSFM)- an emerging and attractive technique for 3D optical sectioning of large samples- appears to be a particularly well-suited approach to deal with them. In this work, we compared the effectiveness of different light sheet illumination modalities reported in the literature to improve resolution and/or light exposure for complex 3D samples. In order to provide an acute and fair comparative assessment, we also developed a systematic, computerized benchmarking method. The outcomes of our experiment provide meaningful information for valid comparisons and arises the main differences between the modalities when imaging different types of TMs.

Spectral degree of linear polarization of light from healthy skin and melanoma.

A non-invasive optical technique, based on a supercontinuum laser source and hyperspectral sensors, is established to measure the spectral degree of linear polarization (DOLP) in a broad spectral range from 525 nm to 1000 nm. Several biomaterials of interest, such as healthy and cancerous skins, are considered. The spectral DOLP of melanoma, from 5 mm to 9 mm diameter, are measured and analyzed. An increase of the spectral DOLP is reported for 100% of the melanoma samples compared to healthy skin samples. The spectral DOLP of a given melanoma appears to be correlated to the stage of its development: the larger the melanoma, the higher the DOLP. Such trend could be explained by a decrease of the surface roughness along the evolution of the disease. In addition, a significant spectral dependence of the DOLP is reported for melanoma samples as it exhibits a decrease in the near infrared from 750 nm to 1000 nm.

Reflectances from a supercontinuum laser-based instrument: hyperspectral, polarimetric and angular measurements.

Recent developments of active hyperspectral systems require optical characterization of man-made materials for instrument calibration. This work presents an original supercontinuum laser-based instrument designed by Onera, The French Aerospace Lab, for fast hyperspectral polarimetric and angular reflectances measurements. The spectral range is from 480 nm to 1000 nm with a 1 nm spectral resolution. Different polarization configurations are made possible in whole spectrum. This paper reviews the design and the calibration of the instrument. Hyper-spectral polarimetric and angular reflectances are measured for reference and man-made materials such as paint coatings. Physical properties of reflectances as positivity, energy conservation and Helmholtz reciprocity are retrieved from measurements.